摘要：Kinetic studies on the inhibition of phospholipase A2 (from Agkistrodon halys blomhoffii) activity with cadmium ion demonstrated that cadmium ion inhibits the enzymic activity competitively with respect to calcium ion and phosphatidylcholine. The Km value was 6.3×10-3M for phosphatidylcholine and the Ki value of cadmium ion was 1.4×10-4M, while, for the calcium ion, the Km value was 2.8×10-4M and K1 value of cadmium ion was 1.4×10-4M. Cadmium-metallothionein isolated from the liver of cadmium-injected mouse did not show any effect on the phospholipase A2 activity and this fact suggests the protective effect of metallothionein against toxic effect of cadmium on lipid metabolism in living cells.
