摘要：As a hygienic chemical study on the treatment and on the influence of selenium to humans, microdetermination of selenium in various kinds of sample was investigated. Selenium (IV) in the sample was converted to selenium (IV) by heating in hydrochloric acid of concentration above 17%. Treatment of selenium (IV) by heating in hydrochloric acid of concentration above 17%. Treatment of selenium (IV) with 4-chloro-o-phenylenediamine at pH 1 in a boiling water-bath gave 5-chloropiaselenol quantitatively. 5-Chloropiaselenol thus obtained was extracted with benzene at pH 1 and determined by ECD gas charomatography with 15% OV-17 Chromosorb W. High sensitivity of this method enables determination of 0.05 ppm of selenium and the calibration curve is linear up to 0.8 ppm. This microdetermination method is considered to be applicable to various kinds of samples such as river water, food, and animal tissues.
