摘要：Metabolism of 3, 5-dimethylphenyl N-methylcarbamate (Cosban) was examined. Cosban was administered to rabbits during 1 week and urine was collected and extracted daily. From the ether extract under acidic condition, 5-hydroxytoluic acid and (3-carboxy-5-methyl) phenyl N-methylcarbamate were isolated and identified with authentic samples by mixed melting point determination, and infrared and nuclear magnetic resonance spectra. 3, 5-Dimethylphenol was detected by thin-layer chromatography (TLC) and gas chromatography. The aqueous layer left after ether extraction was extracted with a mixed solvent of ether and ethanol (2 : 1) and, from this extract, glucuronides of both 5-hydroxytoluic acid and 3, 5-dimethylphenol, and sulfate of 3, 5-dimethylphenol were obtained. To 100 ml of human urine, 1 mg each of Cosban, (3-carboxy-5-methyl) phenyl N-methylcarbamate, 5-hydroxytoluic acid, and 3, 5-dimethylphenol were added and the mixture was kept overnight. These substances were well separated and detected by silica gel TLC using a developing solvent of hexane-ethyl acetate-formic acid (75 : 25 : 5) and a coloration reagent of 2, 6-dibromoquinone chloroimide.
