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  • 标题:トリクロロメチルチオ誘導体としてのサッカリンの薄層およびガスクロマトグラフィー
  • 本地全文:下载
  • 作者:蒔田 政見 ; 山本 重雄 ; 吉原 恵子
  • 期刊名称:Journal of Health Science
  • 印刷版ISSN:1344-9702
  • 电子版ISSN:1347-5207
  • 出版年度:1973
  • 卷号:19
  • 期号:6
  • 页码:297-301
  • DOI:10.1248/jhs1956.19.297
  • 语种:Japanese
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:

    A method has been developed for the analysis of saccharin by gas chromatography. Saccharin was converted to the trichloromethylthio derivative by treatment with perchloromethylthiol in aqueous alkaline solution. The derivative was extracted with carbon tetrachloride and chromatographed on a 1.5% OV-1 phase supported on Shimalite W at 190° using pyrene as an internal standard. The average recovery of added amount of saccharin from orange juice and canned orange was 99%. A sensitive method for the detection of saccharin by thin-layer chromatography (TLC) of the trichloromethylthio derivative has also been developed. A quantity of 1-2μg of the derivative could be detected on TLC layer by spraying the developed plate with 1% ethanolic solution of resorcinol or 0.06% solution of permanganate in acetone.

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