摘要：For the determination of bromvalerylurea in biological samples, charcoal column chromatography was used to remove lipid contaminant present in the extract. The acetone-extract (100-400 mg) was applied to a column of 1.0 g charcoal, and eluted with 100 ml of hexane-ether-isopropanol (90 : 10 : 1) to separate neutral lipid. Bromvalerylurea was quantitatively recovered by the subsequent elution with 30 ml of acetone-methanol (90 : 10) and submitted to gas chromatographic determination. This treatment could eliminate most part of lipid and improve the recovery of bromvalerylurea. The procedure was applied to the determination of bromvalerylurea in liver, kidney, brain and blood of perorally administrated rat, and the time-courses of bromvalerylurea concentration in these tissues were observed.
