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  • 标题:食品の保存料, 人工甘味料などの衛生化学的研究(第12報) : 食品中の水溶性着色料の検出
  • 本地全文:下载
  • 作者:竹下 隆三 ; 坂上 米次
  • 期刊名称:Journal of Health Science
  • 印刷版ISSN:1344-9702
  • 电子版ISSN:1347-5207
  • 出版年度:1969
  • 卷号:15
  • 期号:2
  • 页码:72-76
  • DOI:10.1248/jhs1956.15.72
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:The application of DEAE-Sephadex to the problem of separating certifiable acid dyes in foods and the detection of the dyes by thin-layer chromatography are described. The dyes were able to be transfered from a food to DEAE-Sephadex by only shaking mixture of the both, even when they were bound to cellulosic of proteinous substrates. The dyes were eluted from a column of the dyes-bound DEAE-Sephadex with the equivolume mixture of 2N sulfuric acid isopropyl alcohol. The dyes concentration in eluate was satisfactory for its detection by silicagel and thin-layer chromatography. The solvent systems for the chromatography were composed of ethyl acetate-methyl alcohol-aqueous ammonia (4.5 : 1 : 0.7, or 3.3 : 1 : 1). These systems were most recomendable for the separation of the tested dyes in this experiment ; the former was suitable for the separation of the xanthen dyes and the later of the others.
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