摘要：The Japanese official method for alkaline phosphatase in milk is Sanders-Seger's method which is so complicated that it can be hardly practised in daily routine. Accordingly, milk technicians hope to have a more simple procedure. The method using p-nitrophenyl phosphate as a substrate was ascertained to be simple and practicable. By means of this method, the presence of raw milk can be easily detected, even when it was diluted 1000-2000 times with pure water. The reagent is composed of 100 ml of buffer solution (Na OAc, NaCl, MgSO4 and barbital Na) and 1.5 mg of sodium p-nitrophenyl phosphate. To discriminate yellow coloration due to the presence of alkaline phosphatase, 1 ml of this reagent is added to 1 ml of the sample for testing.
