摘要：Urine specimens are hydrolyzed with hydrochloric acid and the hydrolyzate is extracted with chloroform to eliminate the contaminants. Thereafter sodium hydroxide solution is gradually added to the aqueous solution to adjust to pH 12 and extracted again with chloroform. Interfering substances can be effectively removed by these operations. After neutralizing with 1N hydrochloric acid, the aqueous layer is adjusted to pH 9 with sodium hydrogen bicarbonate and several drops of 28% ammonia solution, and extracted three times with the same amount of the mixed solvent, chloroform : isopropanol (3 : 1). The extracts are combined, washed with saturated aqueous solution of sodium hydrogen bicarbonate, dehydrated with anhydrous sodium sulfate and filtered. The solvent is removed in vacuo, and residue is separated by silicic acid column chromatography and thin layer chromatography. The extracts from spot area on the thin layer are dissolved in 5% HCI. The determination of each component is performed by UV spectrometry at 285 mμ.
