摘要：Previous study has shown that red-fluorescent product was formed by reaction of vitamin B1 with cupric ion, and could be applied to the detection of vitamin B1. The present report deals with the synthesis and the structure of the product of vitamin B1 with copper, cadmium and zinc, respectively. In order to find the condition for the synthesis of the product, the effect of metal ion on the stability of vitamin B1 was examined by determining vitamin B1. When respective ion of copper (II), cadmium (II) and zinc (II) was reacted with thiolized vitamin B1 at pH 10∼11, vitamin B1 was decomposed only by copper vigorously at 37°and slowly at 0°, but the decomposition of vitamin B1 was hardly observed by any other metal under the experimental conditions. Therefore, their metal salts of vitamin B1 were synthesized as follows : vitamin B1-HCl solution (1 mol) was added into NaOH solution (3 mol) at room temperature, after about 45 minutes, the metal ion solution (1 mol) was added to the mixture at pH 10∼11 under cooling and stirring. The precipitate was recrystallized from iso-butanol or iso-propanol. The molar ratio of metal to vitamin B1 of the crystal was found to be 1 to 2 by chemical analysis. The measurements of U.V. and I.R. spectra of their salts showed the structure of metal chelate.
