摘要：The method for micro quantitative estimation of glucosamine (GA) and N-acetylglucosamine (NAGA) in the mixture was established. On application of the glucosamine estimation method of Levvy, et al., it was ascertained that NAGA could be determined without any obstruction even in the presence of acetylating agent. In this case, the equimolar amount of GA and NAGA gave the equal absorbancy at optimum pH by using the suitable quantities of the buffer and acetic anhydride. Therefore, using the acetylating agent, it is possible to determine the total quantity of GA and NAGA by means of this method. Without the acetylating agent, NAGA can be determined alone by the similar procedure. Thus, the quantity of GA corresponds to the difference of two values.
