摘要：Three different methods have been developed for chemical detemination of Blasticidin S, an anti-phytopathogenic fungal substance. Blasticidin S hydrochloride could be isolated from contaminants by thin-layer chromatography and submitted to determination procedures. Blasticidin S exhibited an intensive absorption at 275 mμ in acidic medium and the calibration curve showed a sufficient linearity for determination. Colorimetry of Blasticidin S was also performed by the color produced with sodium chromotropate and formaldehyde, formed by the oxidative decomposition of the N-methyl group in Blasticidin S with potassium permanganate. On the other hand, Blasticidin S was degraded by heating with hydroxylamine and diazo-coupling reaction of the resulting product with diazosulfanilate was capable of colorimetric determination of Blasticidin S.
