摘要：Applicability of submerged culture was tested in order to obtain a suitable mycelial preparation of Trichophyton gypseum for respiratory study. The submerged culture supplied a very fine mycelial suspension, which could make quantitative handling of the preparation possible, as early as 4 days after inoculation. The suspension thus obtained was found to be more preferable than the slice or bored disc of the mycelial pad obtained by surface growth, in respiratory experiments, because the former, which showed a considerably shorter lag phase in the oxidation of glucose, was permeable to many substrates which were not apparently oxidized by the surface pad preparation, and was susceptible to respiratory inhibitors such as cyanide, azide, iodoacetate, and arsenite.
