摘要：Ketamine·HCl or quinine·HCl forms an ion-association complex with ion association reagents such as tropaeoline 00 and erio green B, and the complex can be extracted into dichloromethane. The concentration of ketamine·HCl or quinine HCl in aqueous solution is determined by measuring the absorbance of extracts at 543 nm (ketamine·HCl) or 637 nm (quinine·HCl). Calibration curve obeyed to Beer's law in the range of 1.82×10-8 to 2.55×10-7 mol/ml (ketamine·HCl) or 2.52×10-9 to 6.30×10-8 mol/ml (quinine·HCl), respectively. The determination procedure is as follows : 1 ml of sample solution, 1 ml of 2.72×10-6 mol/ml (tropaeoline 00) or 2.28×10-6 mol/ml (erio green B) reagent, 2 ml of Britton-Robinson buffer solution, and 5 ml of dichloromethane are placed in a 15 ml of test tube. A mixture is shaken for 3 min mechanically and allowed to stand for 30 min. Absorbance of the dichloromethane layer is measured at 637 nm for the determination of quinine·HCl. For ketamine HCl, to 2 ml of dichloromethane layer, 0.5 ml of 10% hydrochloric acid methanol solution is added, and the absorbance is measured at 543 nm. The proposed method is simple, rapid, and useful for the determination of small amounts of the ketamine·HCl or quinine·HCl. No interference was observed with Ca2+, Mg2+, Fe3+, Ni2+, Cu2+, Co2+, Al8+, Zn2+, NO3-, Cl-, glucose and lactose for the determination of ketamine·HCl or quinine·HCl.
关键词：ion association reagent;ketamine·HCl;quinine·HCl;tropaeoline 00;erio green B
