摘要：This report describes the separation of polynuclear aromatic hydrocarbons (PNAH) by the reversed phase thin-layer chromatography (TLC) on Kieselguhr G impregnated with 10% liquid paraffin using acetonitril·water (13 : 7) as a solvent. The separation patterns of 28 kinds of PNAH by this method were compared with those obtained by the TLC systems using acetylated cellulose and Kieselgel. Distribution of Rb (Rf value relative to benzo [a] pyrene) was 0.57-1.90 for the liquid paraffin-Kieselguhr G, 1.00-2.44 for the acetylated cellulose, and 0.45-1.38 for the Kieselgel. Thin-layer chromatography with liquid paraffin-Kieselguhr G is effective for the separation of PNAH, particularly because of its rapidity.
