摘要：A method is described for the determination of benzo[a]pyrene in a sediment sample based on column chromatographic purification and spectrofluorometric analysis. Benzo[a]pyrene was extracted from sediment samples with n-hexane after refluxing with 10% KOH-90% EtOH. It was then separated from interfering materials by passing the n-hexane extract through silica gel and acetylated cellulose columns. The eluate from the latter column with ethanol-ether-water (4 : 2 : 3) mixture gave the same fluorescence and excitation spectrum as benzo[a]pyrene, and was found to be free from the contaminants originally present, by high-pressure liquid chromatography with UV detection at 254 nm. This procedure for the determination of benzo[a]pyrene was applied to the sediment samples from Hamana Lake, Tagonoura harbor, and paddy field in Shizuoka-city. Benzo[a]pyrene was found at levels varying from 0.0 to 255.2 ppb, with average recovery of 82.4 to 85.1%.
