摘要：The reaction mechanism of mercury with selenium in the plasma of rats simultaneously injected HgCl2 and Na2SeO3 was investigated. Sera collected after independent or simultaneous injection of HgCl2 (10 μmol/kg, i.p. or i.v.) and Na2SeO3 (10 μmol/kg, s.c.) were chromatographed on Sephadex G-200 column. Distribution of mercury and selenium in the serum protein fractions of each injection group was determined by atomic absorption analysis and neutron activation analysis. On the other hand, bovine serum albumin, reduced glutathione, Na2SeO3, and HgCl2 were reacted as the model reaction of mercury with selenium in the plasma. The reaction product of mercury with selenium in this reaction was chromatographed on Sephadex G-200 column and compared with in vivo experimental results. These results suggested that the final reaction product of mercury with selenium in the plasma was a colloidal HgSe, formed by the following two reactions : (1) Mercury from the Hg-binding protein transfers to the Se-binding protein and forms an intermediate such as protein-S-Se-Hg+. (2) The Hg-binding protein reacts with the Se-binding protein and forms an intermediate such as protein-S-Hg-Se-S-protein, or selenium from the Se-binding protein transfers to the Hg-binding protein and forms an intermediate such as protein-S-Hg-Se-. Finally, these intermediates decompose to HgSe and then it converts to colloidal HgSe by association. The latter reaction was proved to occur in the plasma after simultaneous injection of HgCl2 (i.v.) and Na2SeO3 (s.c.).
关键词：mercuric chloride;selenite;gelchromatography of serum;mercury binding serum proteins;selenium binding serum proteins;reaction mechanism of mercury with selenium in plasma;colloidal mercuric selenide;mercury metabolism in response to selenite;selenium metabolism in response to mercury;neutron activation analysis
