摘要：From the result of screening microorganisims which produce lipase, it was found that a species of Pseudomonas produced bile sensitive lipase. The microorganisms were cultivated aerobically and the lipase preparation was obtained from the filtered broth. Pharmaceutical studies were made on the preparation. The optimum pH of this preparation for digestion was 7.5, and the very strong digestive activity was shown between pH 5 and pH 11. The stability of the preparetion was very good between pH 8-11, but was not good in artificial intestinal juice. The powdery lipase preparation was relatively stable at 100°C and retained 55% of the activity after 24 hours at 100°C. It was slightly activated with calcium chloride and sodium chloride, inhibited with copper sulfate, ferric chloride, and activated up to 140% with quiniine sulfate in 10-8 M concentration. The digestive activity of this preparation was inhibited with alkaline protease, but elevated with sodium taurocholate, sodium cholate and diluted bile juice.
