摘要：When subjected to paper electrophoresis under the present conditions (buffer, Theorell buffer solution of pH 9∼11 ; ionic strength, 0.07 ; electric pressure, 300 v ; time, 5 hours) insulin migrated towards the anode by ca. 4 cm., forming a clear band. The insulin band was located by duplicate method, cut out, and eluted with water. The eluate was decomposed by the micro-Kjedahl method and nitrogen in the product determind by azotometry. From the comparison of the results with those of biological assay it was concluded that the present chemical method is applicable for determining the potency of insulin preparations.
