摘要：Toxicological studies of carbonyl compounds derived from oxidized lipids on primary cultured rat hepatocytes were investigated. As carbonyl compounds, saturated aldehyde, α, β-unsaturated aldehyde, glyoxals and malondialdehyde were used. Hepatocytes isolated from male Wistar rats by the collagenase perfusion method were cultured in 35 mm dishes in an atmosphere of 5% CO2-95% air at 37°C. Rat hepatocytes in culture for 24 h were incubated in Williams'E medium containing carbonyl compounds. Hepatotoxicity was estimated as the leakage of hepatic enzymes such as lactate dehydrogenase (LDH) and glutamic-oxalacetic transaminase (GOT) from the cells to medium. LDH and GOT activities were determined by an automatical analyzer. The order of hepatotoxic potency was as follows : glyoxals>α, β-unsaturated aldehydes>saturated aldehydes. Malondialdehyde had not hepatotoxicity. Hepatotoxicity of α, β-unsaturated aldehyde was inhibited by the addition of ascorbic acid and glutathione, whereas that of autoxidized methyl linoleate (peroxide value=2538 meq/kg and carbonyl value=872 mmol/kg) was inhibited by the addition of not only 3-tert-butyl-4-hydroxyanisole (BHA) and N, N'-diphenyl-p-phenylenediamine such as antioxidant, but also ascorbic acid and glutathione. From the above results, it is suggested that both hydroperoxides and α, β-unsaturated aldehydes contribute to the hepatotoxicity of autoxidized methyl linoleates.
关键词：primary cultured rat hepatocyte;α, β-unsaturated aldehyde;leakage of hepatic enzyme;autoxidized linoleate;hepatotoxicity;carbonyl compound
