摘要：In order to investigate enzymatic and non-enzymatic detoxication mechanisms of cyanide in vivo and in vitro, compounds derived from cyanide such as thiocyanate and protein bound thiocyano groups were measured. In addition, high performance liquid chromatographic and gas chromatography-mass spectrometric techniques for the determination of 2-iminothiazolidine carboxylic acid (designated as ITC) have been established and applied to the cyanide poisoning study using experimental animals. Furthermore, the determinations of the compounds related to cyanide as indicated above were performed in 16 fire victims for one year. lethal concentrations of cyanide were detected in 56%, but the sum of the concentrations of cyanide and protein bound thiocyano groups showed that 75% of the victims might be exposed to toxic levels of cyanide. Although ITC could not be detected in any cases supplied for this study, the measurements of the blood levels of not only cyanide but also compounds derived from cyanide, such as protein bound thiocyano groups may be very useful to determine the cause of death in fire victims.
