摘要：The GC-MS and TLC methods for the determination and identification of Δ9-tetrahydrocannabinol-11-oic acid, a major urinary metabolite of Δ9-tetrahydrocannabinol which is one of main constituents of marihuana, were established. Δ9-Tetrahydrocannabinol-11-oic acid was detected on TLC by using Fast Blue BB salt as a color reagent. The metabolite was determined as trimethylsilyl derivative of its methyl ester by using electron impact ionization GC-MS and 5'-nor-Δ8-tetrahydrocannabinol-4'-oic acid as an internal standard. The assay provides excellent sensitivity, reproducibility and specificity with detection limit of 1 ng/ml human urine.
关键词：Δ9-tetrahydrocannabinol;Δ9-tetrahydrocannabinol-11-oic acid;5'-nor-Δ8-tetrahydrocannabinol-4'-oic acid;Fast Blue BB salt;GC-MS;TLC;human urine
