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  • 标题:Separation of Urinary Foaming Material by Bubbling of Urine and by Gelatinizing of Solvent with Foam
  • 本地全文:下载
  • 作者:HIDEO ISONO ; MIHO KOMATSU ; TOSHIHIDE SUZUKI
  • 期刊名称:Journal of Health Science
  • 印刷版ISSN:1344-9702
  • 电子版ISSN:1347-5207
  • 出版年度:1996
  • 卷号:42
  • 期号:4
  • 页码:292-300
  • DOI:10.1248/jhs1956.42.292
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:Urine samples are known to foam and gelatinize solvent when shaken. The present work sought to develop a means of separating and characterizing urinary foaming material. A method was developed which can foam and gelatinize solvent. Human urine, after being filtered at pH 10, was bubbled at pH 4-5. The foam was shaken with n-hexane to gelatinize the solvent. The gelatinized solvent layer was washed with water several times, and dried on a glass plate ; dried material was then scraped off with a razor. A soft powder was obtained which was suggested to contain protein and sugar. The two moved together electrophoretically at the site of α-globulin, and are suggested to be a complex of protein and sugar (glycoprotein).
  • 关键词:foam separation;solvent gelatinization;urinary foaming material;glycoprotein
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