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  • 标题:アセチルアセトン法-高速液体クロマトグラフィーによる油脂中飽和アルデヒド類の定量
  • 本地全文:下载
  • 作者:平山 晃久 ; 鎌田 賢治 ; 笠井 映江
  • 期刊名称:Journal of Health Science
  • 印刷版ISSN:1344-9702
  • 电子版ISSN:1347-5207
  • 出版年度:1994
  • 卷号:40
  • 期号:6
  • 页码:574-581
  • DOI:10.1248/jhs1956.40.574
  • 出版社:The Pharmaceutical Society of Japan
  • 摘要:High-performance liquid chromatographic determination for saturated aliphatic aldehydes, specially formaldehyde, acetaldehyde, 1-propanal, 1-butanal, 1-pentanal and 1-hexanal, were studied using a precolumn derivatization reagent acetylacetone. After the selective condensation of these aliphatic aldehydes to form fluorescent dihydrolutidine derivatives, the separation of these compounds was achieved on a ODS column (150×4 mm i.d.) with a linear gradient system of methanol and water (from 50% methanol to 80% methanol in 30 min). The separated lutidine derivatives were detected with a fluorescence monitor (398 nm for excitation and 488 nm for emmission). Three autoxidized unsaturated fatty acid methyl esters and five autoxidized eddible oils were subsequently assayed for 1-propanal (C3), 1-butanal (C4), 1-pentanal (C5) and 1-hexanal (C6). The peaks of formaldehyde and acetaldehyde were interfered with unknown peaks derived from oxidized lipids. Their detection limits (S/N=2) were 29 ng and 39 ng for 1-propanal and 1-hexanal, respectively. The other carbonyl compounds such as α, β-unsaturated aldehydes and glyoxal derivatives were not interfered by this method.
  • 关键词:acetylacetone method;saturated aliphatic aldehyde;HPLC;fluorometric detection;unsaturated fatty acid methyl ester;edible oil
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