摘要：To search indicator organisms for environmental pollution by volatile chlorinated hydrocarbons (VCHs), 1, 1, 1-trichloroethane (MC), carbon tetrachloride (CTC), trichloethylene (TCE) and tetrachloroethylene (PCE) were tested for their ability of bioconcentration in blue mussel, Mytilus edulis inhabiting chiefly seawater, and killifish, Oryzias latipes inhabiting chiefly freshwater. Extraction of VCHs from blue mussel was carried out by the purge-trap method using a Dean-stark apparatus. In the case of killifish, we developed a new method using and improved purge-trap apparatus because it needs small scale analysis due to the little livestock. The levels of VCHs were determined by ECD-GC. Recoveries of VCHs by this method were 81.1% (MC), 72.7% (CTC), 80.1% (TCE) and 75.8% (PCE). Blue mussel and killifish were exposed to VCHs (5-100 ppb) for 8 d and 21 d, respectively. The bioaccumulation factors for VCHs were 2.95 (MC), 4.52 (TCE) and 25.7 (PCE) for blue mussel, and 2.54 (MC), 3.49 (CTC), 2.71 (TCE) and 13.42 (PCE) for killifish. These results showed that these two aquatic organisms, blue mussel and killifish, have a large bioaccumulation potency of PCE. The monitor of VCHs levels using these organisms may offer an easy way to get some information of water pollution by VCHs.
