摘要：The microdetermination of glyoxal bound to proteins in biological samples has been developed using gas chromatography with an electron capture detector (ECD-GC). The treatment of the glyoxal-bound protein with 4-chloro-o-phenylenediamine resulted in the formation of its derivative, 6-chloroquinoxaline, showing the release of glyoxal from the protein. The use of metaphosphoric acid as a precipitant of the protein is suitable because the precipitant did not inhibit the derivatization and free glyoxal in the precipitated protein can be completely rinsed out with water. The results from Sephadex G-25 gel chromatography coupled with this microdetermination method indicated that glyoxal indeed binds to bovine albumin. The amounts of glyoxal bound to the albumin, fetal bovine serum and rat liver homogenate increased with an increase of glyoxal added.
