摘要：The polycyclic aromatic hydrocarbons (PAHs) extracted by blue-cotton (BC) from river waters were analyzed by reversed-phase high-performance liquid chromatography (HPLC) with a spectro-fluorometric detector. Kanzaki River, an affluent of Yodo River in Osaka Prefecture, showed more serious water pollution in terms of BOD, DO, SS, and the number of E. coli, compared with Kako River in Hyogo Prefecture. Mutagenic activities of blue-cotton adsorbed materials from Kanzaki River were markedly higher than those from Kako River in the Ames test using Salmonella typhimurium TA98 and TA100. The extracts showed potent mutagenic activity to TA98 with S9mix. Ten PAH standards were well separated by the reversed-phase HPLC. Six PAHs were found in water from Kako River, all of which increased in proportion to the weight of BC immersed (0.25-2 g). In Kanzaki River, eight PAHs were detected. The total amount of PAHs in Kako River and Kanzaki River were estimated to be 30 and 101 ng/g BC, respectively. These results showed that both PAHs and mutagens, which were adsorbed to blue-cotton, increased with aggravation of water pollution in river waters. However, the mutagenic activity of the extracts could not be explained by the amount of PAHs detected, which might be attributable to polar derivatives of PAHs.
