摘要：The aim of the present study was to confirm putrefactive formation of 3-methylbutyrylurea (3 M) from α-bromoisovalerylurea (BV). A commercial minced pork sample was spiked with BV and incubated at room temperature (above 25°C) for 1 month in a closed vessel. After the incubation, BV and 3 M were determined by thin-layer chromatography using guaiac gum reagent combined with treatment of the plate with chlorine gas and potassium iodide reagent. A spot of 3 M was detected and increased with the time of putrefaction, accompanied with a decrease of the BV spot. This finding suggested that 3 M was formed from BV in a putrefying environment.
