摘要：For the evaluation of 2-alkenal as a precursor of thiobarbituric acid reactive substance (TBA-RS), 2-alkenal (C3-C9 and 2, 4-hexadienal) was incubated with tert-butyl hydroperoxide (t-BuOOH) at 40°C for 2 hr, Fenton reagent (H2O2/Fe2+) or hypoxanthine-xanthine oxidase (Hx-XOD) in citrate buffer at 37°C for 1 hr. A reaction mixture was made to react with thiobarbituric acid (TBA) in 70% acetic acid at 100°C for 30 min. The amount of red pigment formed from the 2-alkenal greatly increased by addition of t-BuOOH. These results indicate that the red pigment was formed from 2-alkenal by the addition of t-BuOOH during the incubation, but that was formed in the TBA reaction process. Then, the usage of TBA reagent containing Sn2+ (5 μmol), which prevents t-BuOOH oxidation in the TBA reaction, 14.6 and 12.5 mol% of TBA-RS were formed from 2-hexenal and 2, 4-hexadienal by heating with t-BuOOH at 100°C for 60 min, respectively. In Fenton reagent (H2O2/Fe2+), the amount of TBA-RS from 2, 4-hexadienal decreased to 28% during the reaction at 100°C for 60 min. In Hx-XOD, TBA-RS from 2-alkenal (C3, C4 and C6) and 2, 4-hexadienal increased with superoxide oxidation. From the above results, it is suggested that 2-alkenal was decomposed with t-BuOOH oxidation and Hx-XOD system, and thiobarbituric acid reactive substances were formed.
