摘要：Gas chromatographic (GC) separating conditions for 2- and 3- series of prostanoids were investigated by using prostaglandin F2α (PGF2α) and PGF3α as model compounds. The combination of the dimethylisopropylsily (DMIPS) ether derivatives and the capillary column coated with a polar liquid stationary phase of 50%-phenylmethylsilicone enabled to separate them with the difference of about 0.3 methylene unit values. This condition was applied for GC/mass spectrometric analysis of the metabolites of eicosapentaenoic acid (EPA), demonstrating the obvious separation of Δ17-6-keto-PGF1α and 11-dehydrothromboxane B3 from the corresponding 2-series metabolites originated from arachidonic acid.
