摘要：The purity test of the Japanese Pharmacopoeia Absorbent Gauze for fluorescent whitening agents is regulated to perform by a method of irradiating the gauze with ultraviolet rays in a dark place. This method has been reported to have a difficulty in detecting the existence of fluorescent whitening agents in several cases. We, therefore, tried to establish a method to examine the existence of fluorescent whitening agents using physical and chemical methodologies. After cutting the Japanese Pharmacopoeia Absorbent Gauze samples in small pieces, fluorescent whitening agents were extracted by 30% pyridine solution at 100°C for 30 min. We analysed the fluorescent agents using reversed phase TLC, spectrofluorophotometer and HPLC with a fluorescence detector using 8 standard fluorescent whitening agents as controls. Conditions of reversed phase TLC : plate, RP-18W (Merck) ; solvent system, A) acetonitrile-water (1 : 4), B) acetonitrile-methanol-water (3 : 3 : 10), C) methyl ethyl ketone-methanol-water (1 : 1 : 1). Conditions of HPLC with fluorescence detection : column, Wakosil 5C18 (i.d. 4.6×150 mm) ; mobile phase, 0.01M ammonium acetate-acetonitrile (7 : 3) ; flow rate, 1.0 ml/min ; injection volume, 20 μl ; detections, fluorescence detector (excitation 345 nm and emission 435 nm).
关键词：fluorescent whitening agent;Absorbent Gauze;purity test;reversed phase TLC;spectrofluorophotometer;HPLC with fluorescence detector
