摘要：A versatile analytical method using liquid-liquid extraction and GC/MS for nicotine and cotinine in the urine was developed. Concentrations of nicotine and cotinine in the urine from 5 non-smokers and 6 smokers were analyzed by this method. The levels of nicotine and cotinine in the urine of smokers were 17-2200 ng/ml and 120-3200 ng/ml, respectively. On the contrary, their levels in the urine of non-smokers were below the detection limit except persons accepting passive smoking. The variations of the concentrations of nicotine and cotinine and that of mutagenic activity after smoking were similar and reached maxima at 3-6 h after smoking.
