摘要：In order to clarify the origin of mutagenic impurities in the commercial cosmetic Lithol Rubine B (C.I. 15850) and Lithol Rubine BCA (C.I. 15850 : 1), several dye samples were synthesized under various conditions and their ethyl acetate extracts were tested for mutagenic activities in the Ames test. When the amount of diazotized 2-amino-5-methylbenzenesulfonic acid in the diazo solution was more than that of 3-hydroxy-2-naphthalenecarboxylic acid in the coupling solution in terms of the theoretical amount, mutagenic compounds were produced as reaction intermediates or by-products without exception. However, mutagenic compounds were never produced when the amount of the former was less than that of the latter in terms of the theoretical amount. The reaction temperature did not affect the production of mutagenic compounds. The mutagenic compounds in the synthetic Lithol Rubine BCA could be removed from the calcium lake by rinsing with water.
