标题：Simultaneous Determination of Urinary Methamphetamine, Cocaine and Morphine Using a Latex Agglutination Inhibition Reaction Test with Colored Latex Particles
摘要：A latex agglutination inhibition reaction test (LAIRT) for simultaneous determination of urinary methamphetamine, cocaine and morphine, called mixed LAIRT, has been established using colored latex particles. Hapten (p-aminomethamphetamine, benzoylecgonine and 3-carboxymethylmorphine for methamphetamine, cocaine and morphine, respectively) was conjugated with bovine serum albumin (BSA) for an immunogen and with rabbit serum albumin (RSA) for an antigen. The antigen and the anti-drug antibody obtained by multiple injections of immunogen to rabbits were coupled covalently to carboxylated latex particles to prepare antigenlatex and antibody-latex reagents. With a mixed LAIRT using a combination of blue latex reagents for methamphetamine, white latex reagents for cocaine and red latex reagents for morphine, urinary methamphetamine, benzoylecgonine and morphine could be analyzed with a cutoff level of 0.3μg/ml urine for methamphetamine and morphine and 0.1μg/ml urine for benzoylecgonine in a short time (about 20 min). The results of the urine of suspected abusers in Germany and Japan analyzed by a mixed LAIRT were compared with those by the enzyme multiplied immunoassay technique and/or GC-MS. It was concluded that mixed LAIRT is a suitable method for mass screening of urine samples of suspected abusers.
