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  • 标题:Molecular typing of Salmonella enterica serover Enteritidis
  • 本地全文:下载
  • 作者:Kaoru NAKATA ; Jun OGASAWARA
  • 期刊名称:生活衛生
  • 印刷版ISSN:0582-4176
  • 电子版ISSN:1883-6631
  • 出版年度:2003
  • 卷号:47
  • 期号:3
  • 页码:133-138
  • DOI:10.11468/seikatsueisei1957.47.133
  • 出版社:Osaka Urban Living and Health Association
  • 摘要:Salmonella enterica serover Enteritidis (SE) strains isolated in Osaka city were genotyped by pulse field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) methods. Fifty-five isolates from 16 food-borne outbreaks, 2 sporadic isolates and an environmental isolate were analyzed. By PFGE method, they were divided into 8 types. Some isolates from different outbreaks showed identical PFGE patterns. Twenty isolates from 11 food-borne outbreaks and an environmental isolate, which showed 4 PFGE patterns, were further analyzed by more resolutive AFLP method. They were typed into 2 patterns. These results suggest that AFLP method is not suitable for genotyping of SE isolated in Osaka city as PFGE method is not. The possibility that identical clones of SE spread in Osaka city has yet to be elucidated.
  • 关键词:Salmonella Enteritidis;genotyping;PFGE;AFLP
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