摘要：The efficacy of commercial disinfectants used at commercial animal farms such as poultry farms for disinfection of pathogenic organisms was examined for ten strains of Salmonella, Campylobacter, Escherichia coli or Staphylococcus aureus isolates, which easy contaminated poultry products. Most of these strains were isolated from patients suffering from food borne diseases. The principal ingredients of commercial disinfectants used were quaternary ammonium chloride preparation, chlorine preparation, orthodichlorobenzene preparation, iodophor preparation, biguanide preparation and glutaraldehyde preparation. The bacteriocidal effect of the disinfectants was expressed by maximum bacteriocidal concentration (MBC) when bacterium was killed after exposure to physiological saline (PS) including the disinfactant for 10min at 20°C. The MBC of quaternary ammonium chloride preparation against most strains of Salmonella or E. coli was 250ppm, and against most strains of S. aureus and Campylobacter was 63ppm. The MBC of chloride preparation was 4.0ppm against most strains of Campylobacter , and 1.0ppm against most strains of Salmonella and E. coli . Orthodichlorobenzene and glutaraldehyde preparations showed an MBC of 250 to 1, 000ppm against each bacterial strain. MBC of iodophor preparation against most strains used were 16ppm. Biguanide prearation gave an MBC of 1, 000ppm against most strains of Salmonella and E. coli , and 63 to 250ppm against of S. aureus and Campylobacter . The MBC against each strain was generally higher at lower temperatures except for orthodichlorobenzene preparation against Salmonella and S. aureus . The variation of the MBC value obtained by examinations at 10 and 30°C against most strains were within 4 fold. In addition, the MBC was measured in brain heart infusion (BHI) medium, and compared with in PS. The MBC obtained by exposure in BHI including disinfectants were higher than it in PS, and the differences in the values obtained between the two solutions including chlorine preparation were larger than the differences between the values at the other disinfectants. No difference was observed in the tests of quaternary ammonium chloride preparation against S. aureus and Campylobacter , orthodichlorobenzene preparation against Salmonella , and biguanide preparation against Campylobacter . Growth of Salmonella, E. coli , and S. aureus was inhibited in BHI including each disinfectant at a concentration equal to or near the MBCs in BHI, but Campylobacter strains did not grow in the broth including each disinfectant at 16 to 128 fold higher concentrations than the MBC.
