摘要：Mutagenic activities of airborne and diesel particulate extracts were measured by the Ames assay method. The neutral fractions of diesel and airborne particulate extracts were separated into 50 fractions by semipreparative normal phase high-performance liquid chromatography (HPLC) and Ames assay (TA 98, without a S9 mix) were performed each fraction. The prominently mutagenic fractions were ones with elution times approximately coincident with those for 1, 3-, 1, 6- and 1, 8-dinitropyrenes (DNP). These mutagenic fractions were further purified by reversed phase HPLC. By means of capillary gas chromatography and negative ion-chemical ionization mass spectrometry with selected ion-monitoring detection, 1, 3-, 1, 6 and 1, 8-DNP have been identified in the most active fractions of purified elutes. From these results, it would be concluded that 1, 3-, 1, 6-, 1, 8-DNP are the major contributers to the direct-acting mutagenicities of airborne and diesel particulates.
