标题：A scanner-based rhizobox system enabling the quantification of root system development and response of Brassica rapa seedlings to external P availability
出版社：Japan Science and Technology Information Aggregator, Electronic
摘要：Rhizoboxes are soil-root compartments that may well provide the closest naturalistic conditions for studying root systems architectures (RSAs) in controlled environments. Rhizobox-based studies can however lead to mis-estimation of root traits due to poor recovery of roots and loss of fine root features during washing of roots. We used a novel scanner-based rhizobox system to evaluate: (i) RSA traits of Brassica rapa genotypes; (ii) the relationship between root traits recorded from rhizoboxes and those of harvested roots and (iii) genotypic variation of seedlings in response to external P ([P]ext) availability. Brassica rapa genotypes were grown in soil-filled rhizoboxes abutting flatbed scanners and were watered once with either deionised water or a solution of 600 ΜM KH2PO4 to approximately 80% field capacity on a weight basis. Shoot and root P concentrations ([P]shoot and [P]root) of the B. rapa lines grown on different [P]ext were quantified. Visible root length at the surface of rhizoboxes constituted 85% of the total root length recovered from harvested root samples. High P supply induced a strong increase in [P]shoot in all genotypes ( P ext. Total root length was strongly correlated with tissue P content under both low [P]ext ( r = 0.81, P ext ( r = 0.82, P
关键词：Brassica rapa L.;nutrient acquisition;phosphorus;rhizobox;root system architecture;scanner-based phenotyping
