期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2017
卷号：13
期号：3
页码：128-137
DOI：10.19026/ajfst.13.4149
出版社：MAXWELL Science Publication
摘要：The objective of the present study is to develop callus induction and plant regeneration; an experiment was conducted to investigate the effect of growth regulators on callus induction in rice mature embryo culture. In this experiment, callus was initiated from the mature seed scutellumof two Malaysian indica rice, MRQ74 and MR269. Callus was induced using different concentrations of 2, 4-D and BAP on MS medium under dark condition or under 16/8 h photoperiod conditions. For complete plant regeneration, the calli of both varieties were planted for shoot regeneration on MS media supplemented with different concentrations of BAP and NAA, for root regeneration on IBA. The results revealed that the maximum callus inductions in MRQ74 and MR269 were observed in the MS medium containing 3 mg/L 2, 4-D with 0.1 mg/L BAP under dark conditions. Furthermore, our protocol uses mature seeds as the explants in both rice varieties in terms of callus induction. Additionally, green plant production of plants was also observed in all of the media tested for shoot regeneration and MS media supplemented with 3 mg/L of BAP and 0.1 mg/L of NAA was found to be responsible for the recovery of more highly regenerated shoots. Effective root induction (100%) was achieved in a medium containing 1mg/LIBA. The described protocol can be useful in the establishment of an in vitro system for rice that can be a major technique for a genetic transformation system and crop improvement.
