摘要：To investigate the genetic changes in the indigenous population of northern Kyushu Island from the early Jomon to Yayoi periods, mitochondrial DNA from nine human skeletal remains excavated from the Iwashita Cave and Shimo-motoyama rock shelter sites located in Sasebo city was examined using the amplified product length polymorphism (APLP) method and next-generation sequencing analyses. Although mitochondrial DNA could be extracted from two samples, we could not determine the genetic characteristics of the population in this area and their changes from the Jomon to Yayoi periods accurately. However, the average sequencing depths for these samples were 178.73 and 61.02, which indicates the validity of the DNA capture methods used in this study. The individuals who were successfully analyzed belonged to the northwestern Kyushu Yayoi population, which is thought to be a descendant of the Jomon people. Therefore, the current data are expected to be used to speculate the characteristics of the mitochondrial DNA of the Kyushu Jomon population. However, mitochondrial haplogroups were assigned as M7a and D4a1 by APLP analysis, and based on the whole mitochondrial DNA genome sequencing data, the ancestral types were found to be M7a1a4 and D4a1. Haplogroup M7a has been detected at several Jomon sites in Honshu and Hokkaido and is thought to be a typical Jomon haplogroup. On the other hand, the existence of haplogroup D4a, considered to be derived from immigrant Yayoi population at the site suggested that the segregation of the indigenous Jomon people and immigrants in this region was not clear.
关键词：ancient DNA ; mitochondria haplogroup ; Shimo-motoyama rock shelter site ; Jomon ; Yayoi
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