首页    期刊浏览 2024年11月05日 星期二
登录注册

文章基本信息

  • 标题:Multiple-locus variable-number of tandem repeat analysis standardization for community isolates methicilineresistant Staphylococcus aureus study in Paraguay
  • 本地全文:下载
  • 作者:Fátima Rodríguez-Acosta ; Silvina Fernández ; Sol Haim
  • 期刊名称:Duazary
  • 印刷版ISSN:1794-5992
  • 电子版ISSN:2389-783X
  • 出版年度:2017
  • 卷号:14
  • 期号:2
  • 页码:131-140
  • 语种:Spanish
  • 出版社:Universidad del Magdalena
  • 摘要:Staphylococcus aureus is a pathogen that can produce several infections with a wide range of severity and it has the ability to adapt to different tissues. The epidemiology is complex, due to circulation of many different clones worldwide, so the analysis for its identification requires reproducible and high discriminatory power molecular methods. The aim of this study was to standardize the molecular technique multiple-locus variable number of tandem repeat analysis (MLVA) for the genetic variability analysis of S. aureus isolates, previously characterized by pulsed field gel electrophoresis (PFGE). The MLVA was made by PCR amplification of seven VNTR locus (clfA, clfB, sdrC, sdrD, sdrE, sspA y spA). A high level of reproducibility has been reached in the study. The use of isolates previously typified by multi-locus sequence typing (MLST), PFGE, locus spa and cassette SCCmec, allowed to validate the MLVA clusters comparatively. The isolates that were clustered by MLVA as the same isolate, showed the same results by other molecular techniques, and the MLVA can distinguish isolates with identical PFGE patterns. This technique meets all the criteria of a useful molecular typification technique.
国家哲学社会科学文献中心版权所有