标题：Method Validation for Simultaneous Determination of Pesticide Residues in Post-Mortem Samples by High Performance Liquid Chromatography-Ultraviolet Method
出版社：International Medical Journal Management and Indexing System
摘要：In forensic or clinical toxicology correct interpretation of toxicological findings need reliable analytical data. The analytical methods that are to be used for routine analysis need careful method development and thorough validation. Blood, body fluids and certain organs are mostly used in examinations of deaths due to intoxication. However validated methods for the analysis of post-mortem samples, especially tissues are very few. The aim of this work was to validate a simple reversed phase HPLC-UV method for the simultaneous determination of carbamate (propoxur), organophosphorus (malathion, quinalfos, profenofos and chlorpyrifos) and organochlorine (endosulfan) pesticides in post mortem tissue and blood samples. The pesticides incorporated in the method were those commonly encountered in suicidal or homicidal poisonings in India. Prior to analysis, QuEChERS isolation technique was conveniently used to clean the tissue and blood samples. Validation proved the applicability of matrix matched calibration for routine screening of the pesticides in postmortem samples. The working range of all the pesticides showed good linearity with correlation coefficients (r) ranging from 0.9996 to 0.9999.The LOD for all pesticides ranges from 0.37 to 0.70 µg/g in tissue while it varies from 0.14 to 0.35 µg/ml in blood. The limit of quantitation ranges from 1.16 to 2.50 µg/g in tissue and from 0.44 to 1.50 µg/ml in blood. The developed method can be effectively used for routine screening and quantitation of the pesticides belonging to diverse groups which otherwise require different time consuming or costly analytical techniques for confirmation and quantitation.
