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  • 标题:A Rapid Method for the Determination of the Total Conjugated Linoleic Acid (CLA) Encapsulated in β-casein Nanoparticles
  • 本地全文:下载
  • 作者:M.H. Abd El-Salam ; M.N.A. Hassan ; A.M. Abd El-Fattah
  • 期刊名称:American Journal of Food Technology
  • 印刷版ISSN:1557-4571
  • 电子版ISSN:1557-458X
  • 出版年度:2017
  • 卷号:12
  • 期号:2
  • 页码:140-143
  • DOI:10.3923/ajft.2017.140.143
  • 出版社:Academic Journals Inc., USA
  • 摘要:Background: β-casein was separated from different kinds of milk (Buffalo, cow, goat and camel) . Conjugated Linoleic Acid (CLA) was loaded in β-casein micelles. The micellar solution was frozen then lyophilized and stored in refrigerator. Methodology: A rapid method has been developed for the determination of encapsulated (CLA) in nano β-casein particles. A rapid method has been developed for the determination of encapsulated conjugated linoleic acid in nano β-casein particles. The method is based on the direct measurements of the absorbance of a solution of CLA loaded nanoparticles at 233 nm. Results: The determined CLA was highly correlated with initial concentration of CLA used in encapsulation and with results obtained by ethanol extraction. Conclusion: The method offers the advantage of eliminating the need for solvent extraction of entrapped CLA before spectrophotometric measurement.
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