摘要：Background: β-casein was separated from different kinds of milk (Buffalo, cow, goat and camel) . Conjugated Linoleic Acid (CLA) was loaded in β-casein micelles. The micellar solution was frozen then lyophilized and stored in refrigerator. Methodology: A rapid method has been developed for the determination of encapsulated (CLA) in nano β-casein particles. A rapid method has been developed for the determination of encapsulated conjugated linoleic acid in nano β-casein particles. The method is based on the direct measurements of the absorbance of a solution of CLA loaded nanoparticles at 233 nm. Results: The determined CLA was highly correlated with initial concentration of CLA used in encapsulation and with results obtained by ethanol extraction. Conclusion: The method offers the advantage of eliminating the need for solvent extraction of entrapped CLA before spectrophotometric measurement.
