文章基本信息
- 标题：Effect of Tetrahydrozoline on the Permeability of Trabecular Meshwork Cell Monolayer
- 本地全文：下载
- 作者：Hwang, Seok Jin ; Kim, Jae Woo
- 期刊名称：Journal of the Korean Ophthalmological Society
- 印刷版ISSN：0378-6471
- 出版年度：2017
- 卷号：58
- 期号：1
- 页码：69-73
- DOI：10.3341/jkos.2017.58.1.69
- 语种：Korean
- 出版社：The Korean Ophthalmological Society
- 摘要：Purpose
To investigate the effects of tetrahydrozoline (THZ) on the survival of cultured human trabecular meshwork cells (HTMC) and the permeability of HTMC monolayer.
MethodsPrimary cultured HTMC were exposed to an adrenergic agonist (0.01, 0.1, 1.0 or 10 µM THZ) for 1 day and 3 days. Carboxyfluorescein permeability through the HTMC monolayer was measured using Transwell. Cellular viability and nitric oxide (NO) production were assessed using MTT and Griess assays, respectively.
ResultsTHZ did not affect the cellular survival ( p > 0.05) or NO production ( p > 0.05). THZ significantly increased the carboxyfluorescein permeability through the HTMC monolayer in a dose-dependent manner compared with non-exposed control ( p < 0.05) after exposure for 1 and 3 days.
ConclusionsTHZ does not affect the survival of HTMC but decreases the permeability of HTMC monolayer in a dose-dependent manner. Thus, THZ may possibly decrease trabecular outflow.
- 关键词：Permeability; Survival; Tetrahydrozoline; Trabecular meshwork cells
