首页    期刊浏览 2024年10月05日 星期六
登录注册

文章基本信息

  • 标题:High Resolution Quantitative Angle-Scanning Widefield Surface Plasmon Microscopy
  • 本地全文:下载
  • 作者:Han-Min Tan ; Suejit Pechprasarn ; Jing Zhang
  • 期刊名称:Scientific Reports
  • 电子版ISSN:2045-2322
  • 出版年度:2016
  • 卷号:6
  • DOI:10.1038/srep20195
  • 出版社:Springer Nature
  • 摘要:We describe the construction of a prismless widefield surface plasmon microscope; this has been applied to imaging of the interactions of protein and antibodies in aqueous media. The illumination angle of spatially incoherent diffuse laser illumination was controlled with an amplitude spatial light modulator placed in a conjugate back focal plane to allow dynamic control of the illumination angle. Quantitative surface plasmon microscopy images with high spatial resolution were acquired by post-processing a series of images obtained as a function of illumination angle. Experimental results are presented showing spatially and temporally resolved binding of a protein to a ligand. We also show theoretical results calculated by vector diffraction theory that accurately predict the response of the microscope on a spatially varying sample thus allowing proper quantification and interpretation of the experimental results.
国家哲学社会科学文献中心版权所有