期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：19
页码：8525-8530
DOI：10.1073/pnas.0914794107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We report the visualization of NO production using fluorescence in tissue slices of the mouse main olfactory bulb. This discovery was possible through the use of a novel, cell-trappable probe for intracellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties installed onto the fluorescent probe are cleaved by intracellular esterases to yield the corresponding negatively charged, cell-impermeable acids. The trappable probe Cu2(FL2E) and the membrane-impermeable acid derivative Cu2(FL2A) respond rapidly and selectively to NO in buffers that simulate biological conditions, and application of Cu2(FL2E) leads to detection of endogenously produced NO in cell cultures and olfactory bulb brain slices.
