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  • 标题:Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP
  • 本地全文:下载
  • 作者:Shinya Kurata ; Klaus H. Nielsen ; Sarah F. Mitchell
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2010
  • 卷号:107
  • 期号:24
  • 页码:10854-10859
  • DOI:10.1073/pnas.1006247107
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:After each round of protein biosynthesis, the posttermination complex (PoTC) consisting of a ribosome, mRNA, and tRNA must be disassembled into its components for a new round of translation. Here, we show that a Saccharomyces cerevisiae model PoTC was disassembled by ATP and eukaryotic elongation factor 3 (eEF3). GTP or ITP functioned with less efficiency and adenosine 5{gamma}'-({beta
  • 关键词:yeast cytoplasm ; ribosome recycling factor ; ribosomal subunits ; kinetics ; antibiotics
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