期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：33
页码：14745-14750
DOI：10.1073/pnas.1001562107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Lymphoid organs are characterized by a complex network of phenotypically distinct dendritic cells (DC) with potentially unique roles in pathogen recognition and immunostimulation. Classical DC (cDC) include two major subsets distinguished in the mouse by the expression of CD8{alpha}. Here we describe a subset of CD8{alpha}+ DC in lymphoid organs of naive mice characterized by expression of the CX3CR1 chemokine receptor. CX3CR1+ CD8{alpha}+ DC lack hallmarks of classical CD8{alpha}+ DC, including IL-12 secretion, the capacity to cross-present antigen, and their developmental dependence on the transcriptional factor BatF3. Gene-expression profiling showed that CX3CR1+ CD8{alpha}+ DC resemble CD8{alpha}- cDC. The microarray analysis further revealed a unique plasmacytoid DC (PDC) gene signature of CX3CR1+ CD8{alpha}+ DC. A PDC relationship of the cells is supported further by the fact that they harbor characteristic D-J Ig gene rearrangements and that development of CX3CR1+ CD8{alpha}+ DC requires E2-2, the critical transcriptional regulator of PDC. Thus, CX3CR1+ CD8{alpha}+ DC represent a unique DC subset, related to but distinct from PDC. Collectively, the expression-profiling data of this study refine the resolution of previous DC definitions, sharpen the border of classical CD8{alpha}+ and CD8{alpha}- DC, and should assist the identification of human counterparts of murine DC subsets.
