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  • 标题:Ectopic vesicular neurotransmitter release along sensory axons mediates neurovascular coupling via glial calcium signaling
  • 本地全文:下载
  • 作者:Anne Thyssen ; Daniela Hirnet ; Hartwig Wolburg
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2010
  • 卷号:107
  • 期号:34
  • 页码:15258-15263
  • DOI:10.1073/pnas.1003501107
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:Neurotransmitter release generally is considered to occur at active zones of synapses, and ectopic release of neurotransmitters has been demonstrated in a few instances. However, the mechanism of ectopic neurotransmitter release is poorly understood. We took advantage of the intimate morphological and functional proximity of olfactory receptor axons and specialized glial cells, olfactory ensheathing cells (OECs), to study ectopic neurotransmitter release. Axonal stimulation evoked purinergic and glutamatergic Ca2+ responses in OECs, indicating ATP and glutamate release. In axons expressing synapto-pHluorin, stimulation evoked an increase in synapto-pHluorin fluorescence, indicative of vesicle fusion. Transmitter release was dependent on Ca2+ and could be inhibited by bafilomycin A1 and botulinum toxin A. Ca2+ transients in OECs evoked by ATP, axonal stimulation, and laser photolysis of NP-EGTA resulted in constriction of adjacent blood vessels. Our results indicate that ATP and glutamate are released ectopically by vesicles along axons and mediate neurovascular coupling via glial Ca2+ signaling.
  • 关键词:neuron–glia interactions ; olfactory bulb ; olfactory ensheathing cells ; purinergic signaling
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