期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2010
卷号：107
期号：6
页码：2568-2573
DOI：10.1073/pnas.0915000107
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The peritoneal cavity (PerC) is a unique compartment within which a variety of immune cells reside, and from which macrophages (MO) are commonly drawn for functional studies. Here we define two MO subsets that coexist in PerC in adult mice. One, provisionally called the large peritoneal MO (LPM), contains approximately 90% of the PerC MO in unstimulated animals but disappears rapidly from PerC following lipopolysaccharide (LPS) or thioglycolate stimulation. These cells express high levels of the canonical MO surface markers, CD11b and F4/80. The second subset, referred to as small peritoneal MO (SPM), expresses substantially lower levels of CD11b and F4/80 but expresses high levels of MHC-II, which is not expressed on LPM. SPM, which predominates in PerC after LPS or thioglycolate stimulation, does not derive from LPM. Instead, it derives from blood monocytes that rapidly enter the PerC after stimulation and differentiate to mature SPM within 2 to 4 d. Both subsets show clear phagocytic activity and both produce nitric oxide (NO) in response to LPS stimulation in vivo. However, their responses to LPS show key differences: in vitro, LPS stimulates LPM, but not SPM, to produce NO; in vivo, LPS stimulates both subsets to produce NO, albeit with different response patterns. These findings extend current models of MO heterogeneity and shed new light on PerC MO diversity, development, and function. Thus, they introduce a new context for interpreting (and reinterpreting) data from ex vivo studies with PerC MO.
