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  • 标题:Spatiotemporal control of spindle midzone formation by PRC1 in human cells
  • 本地全文:下载
  • 作者:Changjun Zhu ; Eric Lau ; Robert Schwarzenbacher
  • 期刊名称:Proceedings of the National Academy of Sciences
  • 印刷版ISSN:0027-8424
  • 电子版ISSN:1091-6490
  • 出版年度:2006
  • 卷号:103
  • 期号:16
  • 页码:6196-6201
  • DOI:10.1073/pnas.0506926103
  • 语种:English
  • 出版社:The National Academy of Sciences of the United States of America
  • 摘要:We have examined the role of PRC1, a midzone-associated, microtubule bundling, Cdk substrate protein, in regulating the spatiotemporal formation of the midzone in HeLa cells. Cdk-mediated phosphorylation of PRC1 in early mitosis holds PRC1 in an inactive monomeric state. During the metaphase-to-anaphase transition, PRC1 is dephosphorylated, promoting PRC1 oligomerization. Using time-lapse video microscopy, RNA interference, 3D immunofluorescence reconstruction imaging, and rescue experiments, we demonstrate that the dephosphorylated form of PRC1 is essential for bundling antiparallel, nonkinetochore, interdigitating microtubules to establish the midzone that is necessary for cytokinesis. Our results thus indicate that PRC1 is an essential factor in controlling the spatiotemporal formation of the midzone in human cells.
  • 关键词:Cdk phosphorylation ; mitosis/cytokinesis ; microtubule-associated proteins ; microtubule bundling
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